Nonexponential kinetics of a single tRNA Phe molecule under physiological conditions
Open Access
- 22 July 1997
- journal article
- research article
- Published by Proceedings of the National Academy of Sciences in Proceedings of the National Academy of Sciences
- Vol. 94 (15) , 7932-7936
- https://doi.org/10.1073/pnas.94.15.7932
Abstract
The fluorescence decay functions of individual, specifically labeled tRNAPhe molecules exhibit nonexponential character as a result of conformational dynamics occurring during the measurement on a single molecule. tRNAPhe conformational states that interchange much more slowly are evidenced by the distribution of lifetimes observed for many individual molecules. A structural model for the nonexponential decay indicates that the tRNAPhe–probe adduct fluctuates between two states, one of which provides conditions that quench the probe fluorescence.Keywords
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