THE REMOVAL OF NON-SPECIFIC COMPONENTS FROM THE SOLUBLE ANTIGENS OF INFLUENZA AND MUMPS VIRUSES

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Abstract
An investigation has been made of methods to remove the non-specific complement-fixing components which sometimes occur in the routine preparation of virus soluble antigens. It was found possible to remove such components from influenza soluble antigens by (1) centrifugation for 1 hour at 36,000 g or (2) treatment of the antigen with 10% Norit, followed by uf. filtration. With mumps soluble antigen, this purification was accomplished by (1) centrifugation as above or (2) heating the antigen for 30 minutes at 70 °C. at pH 9, followed by centrifugation at 9000 g.

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