Comparison of two digestion methods for the determination of selenium in biological samples

Abstract
Two digestion methods for the determination of selenium in biological samples have been compared: a classic digestion in a heating block by means of wet acid-ashing (HNO3–HClO4) and a microwave digestion in an open-vessel system by heating under reflux with HNO3–H2O2. The two methods were used to determine Se in plasma samples and different reference materials. There was no difference between the mean results, and the correlation coefficient for wet acid-ashing versus microwave digestion was 0.94. The two methods yielded accurate results for the certified materials analysed.

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