Differential Expression of Chitinase and ß-1,3-Glucanase Genes in Various Tissues of Potato Plants

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Abstract
We have analyzed the tissue-specific expression of chitinase, β-1,3-glucanase and, for com parison, phenylalanine ammonia-lyase genes in healthy, unstressed potato (Solanum tuberosum) plants by using RNA -RNA hybridization and immunostaining techniques to localize the respective mRNAs and proteins in situ. Preparations of leaf, flower, shoot tip, node and axillary bud revealed that chitinase exhibited a distinctive and characteristic expression pattern in being confined to the epidermal cell layer, while β-1,3-glucanase and phenylalanine ammonia-lyase were present to varying extents in most tissues of these organs. These distinctive patterns of expression suggest fundamentally different roles for the two glucanohydrolases in the metabolism of unstressed plants

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