Acidic Metabolic Products of Polyporus sulphureus

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Abstract
P. Bulphureus produced non-volatile acid equivalent to 6 ml of normal acid per 100 ml of yeast extract medium during 50 days of incubation, while maximum volatile acidity occurred on the 23rd day equivalent to 1.2 ml normal acid, dropping to near zero thereafter. Yields of about one-third of these were obtained on a chemically defined medium. Crystalline precipitates from concentrated culture medium were found to be principally oxalic acid. A partition coefficient indicated acetic acid as the principle volatile acid. Fractionatior. of the acidic material on a silicic acid adsorption column substantiated the presence of oxalic and acetic acids and indicated 7 other acids. The peak effluent volumes of 4 of these acids corresponded to those of malic, fumaric, succinic and citric acids.