Isolation by Differential and Zonal Centrifugation of Minicells Segregated by Escherichia coli
- 1 April 1979
- journal article
- research article
- Published by Microbiology Society in Journal of General Microbiology
- Vol. 111 (2) , 387-397
- https://doi.org/10.1099/00221287-111-2-387
Abstract
Summary: Minicells segregated from Escherichia coli x925 carrying a drug-resistance plasmid were separated from nucleated cells by differential centrifugation and purified by rate-zonal centrifugation in sucrose gradients. Minicells purified in this way were capable of donating the plasmid to nucleated cells. They also incorporated thymidine, uridine and methionine into macromolecules. Methods are described for purification of plasmid-containing mini-cells on a scale large enough to allow isolation of DNA, DNA polymerase and RNA polymerase in sufficient quantities for studies of enzymes involved in replication and transcription of plasmid DNA.Keywords
This publication has 4 references indexed in Scilit:
- A simple method for the production of accurate linear gradients using a constant-speed peristaltic pumpAnalytical Biochemistry, 1968
- Lysis of Escherichia coli with a neutral detergentBiochimica et Biophysica Acta (BBA) - Nucleic Acids and Protein Synthesis, 1967
- MINIATURE escherichia coli CELLS DEFICIENT IN DNAProceedings of the National Academy of Sciences, 1967
- The relation of resistance transfer factors to the F-factor (sex-factor) ofEscherichia coliK12Genetics Research, 1966