Coumarin in Chromosome Analysis
- 1 January 1953
- journal article
- research article
- Published by Taylor & Francis in Stain Technology
- Vol. 28 (5) , 255-257
- https://doi.org/10.3109/10520295309105242
Abstract
Root tips of monocotyledons were soaked 2.5-3.0 hours at 25–27° C. in saturated aqueous coumarin solution and stained in a mixture of N HC 1 and 2% aceto-orcein (1:9 by volume) 3-4 seconds over a flame. They were then squashed in 1% orcein under a cover glass, the excess stain blotted and the cover sealed. Preparations could be kept about one week. Good chromosome morphology was secured.Keywords
This publication has 1 reference indexed in Scilit:
- Permanent Mounts Of Chromosomes After 8-Oxyquinoline and SquashingStain Technology, 1952