Enzymatic reduction of oxidized alpha-1-proteinase inhibitor restores biological activity.
- 1 December 1981
- journal article
- research article
- Published by Proceedings of the National Academy of Sciences in Proceedings of the National Academy of Sciences
- Vol. 78 (12) , 7483-7486
- https://doi.org/10.1073/pnas.78.12.7483
Abstract
The major serum inhibitor of proteolytic activity, .alpha.-1-proteinase inhibitor (.alpha.-1-PI), (or .alpha.-1-antitrypsin) can be readily inactivated by oxidation. This inactivation [implicated in lung tissue destruction in emphysema] appears to be due to the oxidation of a critical methionine(s) in .alpha.-1-PI that is required for the inhibition of [dog neutrophil] elastase activity. An enzyme from Escherichia coli that reduces methionine sulfoxide residues in protein can restore the biological inhibitory activity of canine oxidized .alpha.-1-PI.Keywords
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