Relationships between conformation of antigen and specificity of antibody in radioimmunoassay for steroid hormone. III. Highly specific cortisol-antibody against 6.ALPHA.- and 6.BETA.-hydroxycortisol 6-hemisuccinate-bovine serum albumin conjugates.

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Abstract
Anti-6.alpha.- and anti-6.beta.-Hydroxycortisol 6-hemisuccinate-BSA [bovine serum albumin] antisera (anti-F-6.alpha.-HS and anti-F-6.beta.-HS) were obtained by immunizing rabbits. The specificity of each antiserum was assessed by determining the cross-reactivities with 37 kinds of steroids and was compared with that of anti-cortisol 21-hemisuccinate-BSA conjugate antiserum (anti-F-21-HS). anti-F-6.beta.-HS had higher specificity than that of anti-F-6.alpha.-HS for the steroids structurally different to cortisol in ring A. anti-F-21-HS had an obviously lower specificity than those of anti-F-6.alpha.-HS and anti-F-6.beta.-HS for the steroids differing structurally from cortisol only in the 17-side chain.