Separation of Hematopoietic Progenitor Cells from Human Umbilical Cord Blood
- 1 January 1993
- journal article
- research article
- Published by Mary Ann Liebert Inc in Journal of Hematotherapy
- Vol. 2 (2) , 243-245
- https://doi.org/10.1089/scd.1.1993.2.243
Abstract
Human umbilical cord blood (HUCB) is a rich source of hematopoietic progenitor cells (HPC). Nevertheless, it has been previously reported that attempts to enrich for HPC resulted in substantial loss of progenitor cells. We have developed a cell processing technique for HUCB using 3% gelatin sedimentation and the AIS MicroCELLector™SBA and CD34. By this technique, we were able to deplete red blood cells (RBC) and obtain a partial T cell depletion, while maintaining 70-90% of the HPC. The results indicate that it may be possible to manipulate cord blood without significant loss of HPC, thus enhancing its utility for transplantation.Keywords
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