Confirmation of the Fur operator site by insertion of a synthetic oligonucleotide into an operon fusion plasmid
Open Access
- 31 January 1988
- journal article
- research article
- Published by American Society for Microbiology in Journal of Bacteriology
- Vol. 170 (2) , 1015-1017
- https://doi.org/10.1128/jb.170.2.1015-1017.1988
Abstract
We constructed a synthetic oligonucleotide corresponding to the previously proposed consensus binding site for the Fur protein, a central iron-regulatory protein of Escherichia coli. When this oligonucleotide was introduced at the start of transcription of an operon fusion between the ompF promoter and the lacZ structural gene, beta-galactosidase activity became iron regulated. This consensus sequence is sufficient to function as an operator site for the binding of Fur protein in vivo.This publication has 26 references indexed in Scilit:
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