Isolation of a brain peptide identical to the intestinal PHI (peptide HI)

Abstract
The isolation of a brain peptide identical to the intestinal peptide PHI (peptide HI) is described. The peptide was isolated from porcine brain extract using a chemical assay method based on its C‐terminal isoleucine amide structure. The complete amino acid sequence of the peptide was found to be: This sequence is identical to the intestinal peptide thus demonstrating PHI to be a brain‐gut peptide. The role of PHI in the central nervous system as a neurotransmitter or neuromodulator is discussed.