Determination of Tryptophan with HPLC after Alkaline Hydrolysis in Autoclave using α-methyl-tryptophan as Internal Standard
- 1 January 1991
- journal article
- research article
- Published by Taylor & Francis in Acta Agriculturae Scandinavica
- Vol. 41 (3) , 305-309
- https://doi.org/10.1080/00015129109439913
Abstract
A method for determination of tryptophan in feeding stuffs is described. The hydrolysis is carried out on a sample containing 10 mg nitrogen using 25 ml NaOH 4.2 mol/l at 110°C for 16 hours in an autoclave. α-methyl-tryptophan is used as internal standard. The tryptophan and the internal standard are determined by using HPLC with fluorescence detection. When analysing three known proteins the number of Trp-residues found was in good agreement with their known numbers. A good mixture linearity response for three sets of mixtures of feeding stuffs is shown.Keywords
This publication has 2 references indexed in Scilit:
- Tryptophan determination of food proteins by h.p.l.c. after alkaline hydrolysisJournal of the Science of Food and Agriculture, 1985
- Hydrolysate preparation for amino acid determinations in feed constituentsZeitschrift für Tierphysiologie Tierernährung und Futtermittelkunde, 1980