INSULIN REGULATES PLACENTAL LACTOGEN AND ESTRADIOL SECRETION BY CULTURED HUMAN TERM TROPHOBLAST

Abstract
We have described a human term trophoblast cell culture system which synthesizes hormones de novo from their precursors. In the present report we utilized this monolayer system to show that insulin produced a dose-dependent stimulation of hPL secretion, with a significant effect at 10−10 M insulin. At 10−9 M concentration, insulin also inhibited estradiol secretion by the cultured trophoblast. We conclude that insulin regulates secretion of estradiol and placental lactogen by cultured human term trophoblast.

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