Detecting Single Stranded DNA with a Solid State Nanopore
Top Cited Papers
- 31 August 2005
- journal article
- letter
- Published by American Chemical Society (ACS) in Nano Letters
- Vol. 5 (10) , 1905-1909
- https://doi.org/10.1021/nl051199m
Abstract
Voltage biased solid-state nanopores are used to detect and characterize individual single stranded DNA molecules of fixed micrometer length by operating a nanopore detector at pH values greater than ∼11.6. The distribution of observed molecular event durations and blockade currents shows that a significant fraction of the events obey a rule of constant event charge deficit (ecd) indicating that they correspond to molecules translocating through the nanopore in a distribution of folded and unfolded configurations. A surprisingly large component is unfolded. The result is an important milestone in developing solid-state nanopores for single molecule sequencing applications.Keywords
This publication has 21 references indexed in Scilit:
- Probing Single DNA Molecule Transport Using Fabricated NanoporesNano Letters, 2004
- DNA molecules and configurations in a solid-state nanopore microscopeNature Materials, 2003
- Nanopore sensors for nucleic acid analysisJournal of Physics: Condensed Matter, 2003
- Fabrication of solid-state nanopores with single-nanometre precisionNature Materials, 2003
- Dynamics of polynucleotide transport through nanometre-scale poresJournal of Physics: Condensed Matter, 2003
- Ion-Beam Sculpting Time ScalesPhysical Review Letters, 2002
- Charged polymer membrane translocationThe Journal of Chemical Physics, 2002
- Driven DNA Transport into an Asymmetric Nanometer-Scale PorePhysical Review Letters, 2000
- Microsecond Time-Scale Discrimination Among Polycytidylic Acid, Polyadenylic Acid, and Polyuridylic Acid as Homopolymers or as Segments Within Single RNA MoleculesPublished by Elsevier ,1999
- Counting polymers moving through a single ion channelNature, 1994