The Enzymic Preparation of Diphosphoinositides
- 1 January 1977
- journal article
- research article
- Published by Taylor & Francis in Preparative Biochemistry
- Vol. 7 (6) , 457-465
- https://doi.org/10.1080/00327487708065513
Abstract
A procedure for the preparation of diphosphoinositides is described. Triphosphoinositides isolated from bovine brain are hydro-lysed by the triphosphoinositide phosphatase (EC 3.1.3.36) from Crithidia fasciculata in the presence of MgCl2 and cetyltrimethyl-ammonium bromide. The diphosphoinositides produced are not degraded further and can be recovered from the reaction mixture in greater than 80% yield. The product is chromatographically pure and has the same structure (1-phosphatidylinositol 4-phosphate) as naturally occurring diphosphoinositides.This publication has 17 references indexed in Scilit:
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