Functional expression of human P‐glycoprotein in Schizosaccharomyces pombe
Open Access
- 20 September 1993
- journal article
- Published by Wiley in FEBS Letters
- Vol. 330 (3) , 279-282
- https://doi.org/10.1016/0014-5793(93)80888-2
Abstract
Human MDR1 cDNA was introduced into the human cultured cells KB‐3‐1 and Schizosaccharomyces pombe pmdI null mutant KN3. The drug sensitivity of KB‐G2 and KN3/pgp, expressing human P‐glycoprotein, was examined. KB‐G2 was resistant to the peptide antibiotics valinomycin and gramicidin D as well as having a typical multidrug resistance (MDR) phenotype. KN3/pgp was resistant to valinomycin and actinomycin D, but not to adriamycin. The ATP‐hydrolysis‐deficient mutant did not confer KN3 resistance to these antibiotics. Human P‐glycoprotein expressed in S. pombe seemed to lack N‐glycosylation. The N‐glycosylation‐deficient mutant, however, conferred a typical MDR phenotype on KB‐3‐1. These results suggest that human P‐glycoprotein functions as an efflux pump of valinomycin and actinomycin D in the membrane of S. pombe.Keywords
This publication has 15 references indexed in Scilit:
- Human P-glycoprotein transports cortisol, aldosterone, and dexamethasone, but not progesterone.Published by Elsevier ,2021
- Increased accumulation of drugs in a multidrug resistant cell line by alteration of membrane biophysical propertiesBiochimica et Biophysica Acta (BBA) - Molecular Cell Research, 1993
- Specific drug binding by purified lipid-reconstituted P-glycoprotein: Dependence on the lipid compositionBiochimica et Biophysica Acta (BBA) - Biomembranes, 1992
- Functional Complementation of Yeast ste6 by a Mammalian Multidrug Resistance mdr GeneScience, 1992
- Functional expression of human mdr1 in the yeast Saccharomyces cerevisiae.Proceedings of the National Academy of Sciences, 1992
- A leptomycin B resistance gene of Schizosaccharomyces pombe encodes a protein similar to the mammalian P‐glycoproteinsMolecular Microbiology, 1992
- Discrete mutations introduced in the predicted nucleotide-binding sites of the mdr1 gene abolish its ability to confer multidrug resistance.Molecular and Cellular Biology, 1989
- P-Glycoprotein gene (MDR1) cDNA from human adrenal: Normal P-glycoprotein carries Gly185 with an altered pattern of multidrug resistanceBiochemical and Biophysical Research Communications, 1989
- THE BIOCHEMISTRY OF P-GLYCOPROTEIN-MEDIATED MULTIDRUG RESISTANCEAnnual Review of Biochemistry, 1989
- Internal duplication and homology with bacterial transport proteins in the mdr1 (P-glycoprotein) gene from multidrug-resistant human cellsCell, 1986