Isolation and Identification of a Cardioactive Peptide fromTenebrio molitorandSpodoptera eridania
- 1 January 1993
- journal article
- Published by Walter de Gruyter GmbH in Biological Chemistry Hoppe-Seyler
- Vol. 374 (7-12) , 1065-1074
- https://doi.org/10.1515/bchm3.1993.374.7-12.1065
Abstract
We isolated several cardioactive peptides from extracts of whole heads of the mealworm, Tenebrio molitor, and the southern armyworm, Spodoptera eridania, using a semi-isolated heart of Manduca sexta for bioassay. We have now isolated from each species the peptide with the strongest effect on rate of contraction of the heart. The peptides were identified using micro Edman sequencing and mass spectrometric methods. This cardioactive peptide has the same primary structure from both species: Pro-Phe-Cys-Asn-Ala-Phe-Thr-Gly-Cys-NH2, a cyclic nonapeptide which is identical to crustacean cardioactive peptide (CCAP) originally isolated from the shore crab, Carcinus maenas, and subsequently isolated from Locusta migratoria and Manduca sexta. This is additional evidence that CCAP has widespread occurrence in arthropoda.Keywords
This publication has 18 references indexed in Scilit:
- Presence of myotropins in larval midgut extracts of lepidopteran insects: Manduca sexta, Agrotis segetum and Spodoptera examptaJournal of Insect Physiology, 1992
- Proctolin: A review with emphasis on insectsJournal of Neurobiology, 1989
- Identification and distribution of a proctolin‐like neuropeptide in the nervous system of the gypsy moth, Lymantria dispar, and in other lepidopteraJournal of Comparative Neurology, 1989
- Isolation, primary structure and bioactivity of schistoflrf-amide, a FMRF-amide-like neuropeptide from the locust, Schistocerca gregariaBiochemical and Biophysical Research Communications, 1989
- Occurrence of crustacean cardioactive peptide (CCAP) in the nervous system of an insect,Locusta migratoriaJournal of Comparative Physiology B, 1989
- Isolation and primary structure of a neuropeptide hormone from Heliothis zea with hypertrehalosemic and adipokinetic activitiesBiochemical and Biophysical Research Communications, 1988
- Hyperglycaemic and myoactive factors in the corpora cardiaca of the mealworm, Tenebrio molitorJournal of Insect Physiology, 1988
- Unusual cardioactive peptide (CCAP) from pericardial organs of the shore crab Carcinus maenasProceedings of the National Academy of Sciences, 1987
- Isolation and primary structure of two peptides with cardioacceleratory and hyperglycemic activity from the corpora cardiaca of Periplaneta americana.Proceedings of the National Academy of Sciences, 1984
- Chemical Defence in Central American Non-Ant-AcaciasJournal of Animal Ecology, 1973