The viability of deep-frozen cow embryos
- 1 March 1978
- journal article
- research article
- Published by Bioscientifica in Reproduction
- Vol. 52 (2) , 391-393
- https://doi.org/10.1530/jrf.0.0520391
Abstract
Day 7 cow embryos were frozen in 1.5 M-DMSO [dimethylsulfoxide] in PBS [phosphate-buffered saline] at 0.3.degree. C/min to -36.degree. C and at 0.1.degree. C/min between -36 and -60.degree. C before being plunged directly into liquid N. They were subsequently thawed (rapidly to -50.degree. C, at 4.degree. C/min from -50 to -10.degree. C and rapidly again) to room temperature. Embryonic viability was tested by 4 different transfer techniques. Maximum pregnancy rate (8/12) was obtained with surgical transfer immediately after thawing and dilution of DMSO.This publication has 0 references indexed in Scilit: