Monoclonal anti‐cardiolipin antibodies bind to DNA

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Abstract
BALB/c mice immunized with the phospholipid, cardiolipin, produced anti‐cardiolipin and anti‐DNA antibodies. Seven hybridomas derived from spleen cells of the cardiolipin‐immunized mice produced cardiolipin‐binding monoclonal antibodies that also bound to the polynucleotides DNA, poly(dT), and poly(I). The seven cardiolipin‐induced monoclonal antibodies shared idiotypic determinants with a high frequency idiotypic marker of spontaneously expressed anti‐DNA autoantibodies of lupus‐prone MRL‐lpr/lpr mice. The monoclonal antibodies presumably bound to phosphodiester phosphate groups that occur in both polynucleotides and phospholipids. The results imply that production of anti‐DNA autoantibodies does not require immunization by DNA.