Gas Chromatography of Bacterial Whole Cell Methanolysates

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Abstract
Fourteen strains of altogether eight species of neisseriae were “fingerprinted” by gas‐liquid chromatographic (GLC) analysis of their trifluoroacetyl derivatized whole cell methano‐lysates. The elution profiles were compared with each other and with those of previously analysed Moraxella species. The chromatograms of “false neisseriae” (Neisseria catarrhalis, N. ovis and N. caviae) and of cocci belonging to “true neisseriae” (N. cinerea, N. flavescens, N. meningitidis and N. gonorrhoeae examined) formed two relatively homogeneous groups distinctly different from each other. The “false neisseriae” group showed a striking chromatographic resemblance to most species of the present genus Moraxella. The M. kingii profile, however, differing from other moraxellae, also differed from both groups of neisseriae. The recently described rodshaped Neisseria elongata revealed, as expected from nucleic acid compatibility tests, a chromatographic pattern almost completely identical to those of other “true neisseriae” species. The results therefore confirm previous conclusions on group relations in Neisseriaceae based on genetic affinities.