Criteria for selecting monoclonal antibodies with respect to accumulation in melanoma tissue
- 31 March 1987
- journal article
- research article
- Published by Springer Nature in Cancer Immunology, Immunotherapy
- Vol. 24 (2) , 151-157
- https://doi.org/10.1007/bf00205593
Abstract
Immunohistology provides a necessary but insufficient criterion for selecting monoclonal antibodies (MAbs) capable of tumour targeting in vivo. Additional selection procedures have been evaluated using a panel of anti-melanoma MAbs, including immunoreactivity of (labelled) MAbs, antibody affinity, kinetics of binding and release, apparent antigen density and accumulation in nude mouse transplants. According to these criteria, MAbs M.2.7.6 and M.2.9.4 showed the most favourable properties, i.e. high immunoreactivity and pronounced internalization into melanoma cells. With MAbs M.2.10.15 and KG 6–56, moderate immunoreactivity and a binding pattern characterized by temperature dependence in the absence of internalization was observed. According to the paired label assay, all four MAbs showed specific accumulation into solid melanoma tissue. However, application in the patient still requires evaluation of the side effects of antigen cross-expression on normal human tissues.Keywords
This publication has 15 references indexed in Scilit:
- Immunoreactivity of monoclonal anti-melanoma antibodies in relation to the amount of radioactive iodine substituted to the antibody moleculeEuropean Journal of Nuclear Medicine and Molecular Imaging, 1985
- Limiting factors in the localization of tumours with radiolabelled antibodiesImmunology Today, 1985
- Determination of the immunoreactive function of radiolabeled monoclonal antibodies by linear extrapolation to binding at infinite antigen excessJournal of Immunological Methods, 1984
- The expression of tumor-associated antigens in primary and metastatic human malignant melanoma.1984
- Relationship Between Antigen Density and Immunotherapeutic Response Elicited by Monoclonal Antibodies Against Solid Tumors23JNCI Journal of the National Cancer Institute, 1984
- Direct Binding of Radioiodinated Monoclonal Antibody to Tumor Cells: Significance of Antibody Purity and Affinity for Drug Targeting or Tumor ImagingHybridoma, 1983
- Surface antigens of melanocytes and melanomas. Markers of melanocyte differentiation and melanoma subsets.The Journal of Experimental Medicine, 1982
- Distribution and molecular characterization of a cell‐surface and a cytoplasmic antigen detectable in human melanoma cells with monoclonal antibodiesInternational Journal of Cancer, 1981
- Isolation of pure IgG1, IgG2a and IgG2b immunoglobulins from mouse serum using protein A-SepharoseImmunochemistry, 1978
- Protein and cell membrane iodinations with a sparingly soluble chloroamide, 1,3,4,6-tetrachloro-3a,6a-diphenylglycolurilBiochemical and Biophysical Research Communications, 1978