Quantitative Analysis of Alprazolam and Triazolam in Hemolysed Whole Blood and Liver Digest by GC/MS/NICI with Deuterated Internal Standards
- 1 January 1994
- journal article
- research article
- Published by Oxford University Press (OUP) in Journal of Analytical Toxicology
- Vol. 18 (1) , 1-6
- https://doi.org/10.1093/jat/18.1.1
Abstract
This report describes sensitive and specific methods for the quantitation of alprazolam and triazolam in hemolysed whole blood and liver tissue. Samples of blood and enzyme-digested liver are extracted without pH adjustment with n-butyl chloride, after addition of deuterated internal standards and urea. The evaporated extracts are reconstituted in acetonitrile for analysis by gas chromatography/mass spectrometry/negative ion chemical ionization (GC/MS/NICI). Fatty extracts may be cleaned up by partitioning between pentane and acetonitrile. Two ion pairs are monitored for each drug. Within-day coefficients of variation in the range 10–50 µg/L for blood are approximately 5%. Between-day coefficients of variation are less than 10%. The limit of quantitation (based on analysis of 0.2-mL blood samples) is 0.5 µg/L for triazolam and 4 µg/L for alprazolam.Keywords
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