Comparison of Various Procedures for Removing Proteins and Nucleic Acids from Cell Walls of Bacillus Subtilis
- 1 January 1976
- journal article
- research article
- Published by Taylor & Francis in Preparative Biochemistry & Biotechnology
- Vol. 6 (6) , 479-488
- https://doi.org/10.1080/00327487608069130
Abstract
Several procedures were used i n an attempt to prepare clean cell walls from Bacillus subtilis. The results indicate that protein and nucleic acids are tightly bound tothe walls. cleanest wall preparations were found following trichloroacetic acid extraction at 60° or by extraction with 0.lN NaOH under a nitrogen atmosphere for 10 hrs. Protein denaturants, such as sodium dodecyl sulfate and concentrated guanidine hydrochloride were relatively ineffective in removing proteins and nucleic acids from the cell walls. Cell wall-bound DNA was biologically The active i n transformation assays.This publication has 4 references indexed in Scilit:
- Preparation of enzymically active, water-insoluble derivatives of trypsinArchives of Biochemistry and Biophysics, 1967
- The isolation of structural components present in the cell wall of Bacillus licheniformis N.C.T.C. 6346Biochemical Journal, 1965
- TRANSFORMATION OF BIOCHEMICALLY DEFICIENT STRAINS OF BACILLUS SUBTILIS BY DEOXYRIBONUCLEATEProceedings of the National Academy of Sciences, 1958
- PROTEIN MEASUREMENT WITH THE FOLIN PHENOL REAGENTJournal of Biological Chemistry, 1951