SPR Imaging of Photo-Cross-Linked Small-Molecule Arrays on Gold
- 18 February 2006
- journal article
- research article
- Published by American Chemical Society (ACS) in Analytical Chemistry
- Vol. 78 (7) , 2226-2230
- https://doi.org/10.1021/ac051777j
Abstract
Identification of small-molecule ligands for a protein of interest can facilitate the analysis of the protein's functions in biological systems. Small-molecule microarrays have allowed for rapid detection of such ligand−protein interactions in a high-throughput manner, although a label on a protein is needed to observe these interactions. By combining SPR imaging technology with our recently developed photo-cross-linked small-molecule array platform, we developed a novel platform that allows in situ observation of interactions between photo-cross-linked small molecules on gold surfaces and nonlabeled proteins in solution. Interactions of estrogenic and androgenic substances with estrogen receptor α were observed using this platform.Keywords
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