Engraftment and Multilineage Expression of Human Bone Marrow CD34− Cells In Vivoa
- 1 April 1999
- journal article
- Published by Wiley in Annals of the New York Academy of Sciences
- Vol. 872 (1) , 220-232
- https://doi.org/10.1111/j.1749-6632.1999.tb08467.x
Abstract
The fetal sheep competitive engraftment model of human hematopoietic stem cells (HSC) was used to evaluate the in vivo engraftment potential of human bone marrow CD34- Lin- cells. Transplantation of CD34- Lin- cells into primary hosts resulted in the long-term (> 1 year) engraftment and multilineage donor cell/progenitor expression with production of significant numbers of CD34+ cells. Secondary transplantation and limiting dilution studies confirmed the presence in human CD34- fraction of HSC with in vivo long-term engraftment and multilineage differentiation potentials.Keywords
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