Activation of atrial muscarinic K+ channels by low concentrations ofβγ subunits of rat brain G protein
- 1 January 1989
- journal article
- research article
- Published by Springer Nature in Pflügers Archiv - European Journal of Physiology
- Vol. 413 (3) , 325-327
- https://doi.org/10.1007/bf00583550
Abstract
Effects of G protein βγ subunits from rat brain on cardiac K+ channel was examined in single atrial cells of guinea-pig, using patch clamp techniques. We found that 10 pM concentration of rat brain βγ subunits preparation could activate the atrial muscarine receptor-gated K+ channel (IK.ACh). Neither the detergent, CHAPS, used to suspend βγ nor the boiled βγ preparation activated IK.ACh. Furthermore, preincubation of βγ subunits preparation in Mg2+-free solution, which easily inactivated α-GTP-γS, did not affect βγ-activation of IK.ACh. We concluded, therefore, that βγ subunits themselves can activate IK.ACh.This publication has 18 references indexed in Scilit:
- Specificity of action of guanine nucleotide-binding regulatory protein subunits on the cardiac muscarinic K+ channel.Proceedings of the National Academy of Sciences, 1988
- Stimulation of phospholipase A2 activity in bovine rod outer segments by the beta gamma subunits of transducin and its inhibition by the alpha subunit.Proceedings of the National Academy of Sciences, 1987
- G protein opening of K+ channelsNature, 1987
- The α Subunit of the GTP Binding Protein G k Opens Atrial Potassium ChannelsScience, 1987
- A new GTP‐binding protein in brain tissues serving as the specific substrate of islet‐activating protein, pertussis toxinFEBS Letters, 1987
- Direct Activation of Mammalian Atrial Muscarinic Potassium Channels by GTP Regulatory Protein G kScience, 1987
- The βγ subunits of GTP-binding proteins activate the muscarinic K+ channel in heartNature, 1987
- Role of intracellular Mg2+ in the activation of muscarinic K+ channel in cardiac atrial cell membranePflügers Archiv - European Journal of Physiology, 1986
- On the mechanism of activation of muscarinic K+ channels by adenosine in isolated atrial cells: involvement of GTP-binding proteinsPflügers Archiv - European Journal of Physiology, 1986
- Uncoupling of cardiac muscarinic and β-adrenergic receptors from ion channels by a guanine nucleotide analogueNature, 1985