Development of blastomeres of mouse eggs isolated at the 4- and 8-cell stage

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Abstract
Organization of a mammahan egg at the beginning of development has been the subject of several experimental as well as descriptive cytological and cytochemical investigations. The regulative capacities of blastomeres isolated at the 2-cell stage have been studied in the rat (Nicholas & Hall, 1942), rabbit (Seidel, 1952,1960) and mouse (Tarkowski, 1959a, b; Mulnard, 1965a, b). Investigations by Seidel and Tarkowski have shown that although the majority of 1/2 blastomeres can regulate and develop into smaller but otherwise normal blastocysts, som e of them give rise to purely trophoblastic vesicles devoid of the inner cell mass. In the mouse, Tarkowski (1959 a, b) observed in addition in a few cases multicellular morulae which had not undergone cavitation. The findings of Tarkowski have been recently confirmed by Mulnard (1965 a, b) who studied cytochemically mouse 1/2 blastomeres developing in vitro.