Scanning and Transmission Electron Microscopy of Candida albicans Chlamydospores

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Abstract
A simple, convenient method of growing large quantities of C. albicans chlamydospores on a cellulose dialysis membrane was developed. Long, narrow, cylindrical suspensor cells bearing spherical to ovoid chlamydospores were observed. Ultrastructural observations showed the chlamydospore to have a bilayered cell wall made up of an outer electron-transparent primary layer and an inner electron-dense secondary layer, a large portion of the total cell volume occupied by a single large vacuole and several smaller vacuoles, and cytoplasmic organelles typical of those observed in the yeast-like cell. There are structural similarities between the region of chlamydospore-suspensor cell connection and septa observed in budding yeast-like cells.

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