Intracellular volume of osmotically regulated C-6 glioma cells

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Abstract
The intracellular volume of neoplastic brain cells was investigated with regard to the effects of hypo‐osmolality and hyperosmolality utilizing double isotopic labeling with 3–0‐methyl‐D‐glucose or tritiated water to measure the total volume of the pellet and inulin or polyethyleneglycol to measure the extracellular volume of the pellet. The cellular pellets were rapidly separated from the incubation medium by centrifugation after addition of an oil mixture. After 60 minutes incubation in Hanks balanced salt medium, the intracellular volume was 7.50 ± 0.64, 8.48 ± 0.19, and 2.97 ± 0.18 ml H2O per 106 packed cells for C‐6 glioma cells, N18TG‐2 neuroblastoma cells, and NG108‐15 neuroblastoma X glioma hybrid cells, respectively. The extracellular trapped space of these cultured cells was about one third of the intracellular volume. The intracellular volume of C‐6 glioma cells was increased in hypotonic environment, whereas it was decreased with hyperosmolality. Both intracellular sodium and potassium were increased with increased osmolality of the incubation media. These data indicate iso‐osmotic regulation by tumor cells, i.e., there is a good correlation between the intracellular volume, intracellular cations and lactate levels of C‐6 glioma cells under various osmotic conditions.