Purification and Characterization of a Human Leukemia Cell-Derived Immunosuppressive Factor
- 1 March 1991
- journal article
- research article
- Published by Taylor & Francis in Preparative Biochemistry
- Vol. 21 (1) , 25-33
- https://doi.org/10.1080/10826069108021513
Abstract
A human leukemia cell-derived suppressor factor (LDSF) capable of suppressing in vitro proliferation and activation of normal human lymphocytes was purified from human leukemic HL-60 cells. LDSF is constitutively produced by the cells and was purified from serum free culture supernatant by a combination of ion-exchange chromatography, gel filtration and electrophoresis. Purified LDSF was determined to be a single chain protein with an apparent molecular mass of 66,000 daltons. LDSF was not cytolytic to lymphocytes, was heat stable at 70[ddot] C, and did not have any effect on IL-2 or transferrin receptor expression.Keywords
This publication has 16 references indexed in Scilit:
- Purification of A Suppressor Lymphokine (SL) from a Human T-Cell LineImmunological Investigations, 1989
- A suppressor lymphokine produced by human T leukemia cell lines. Partial characterization and spectrum of activity against normal and malignant hemopoietic cells.The Journal of Experimental Medicine, 1986
- A mutant human T-cell line producing immunosuppressive factor(s)Cellular Immunology, 1984
- Biosynthesis of ferritin subunits from different cell lines of HL‐60 human promyelocytic leukaemia cells and the release of acidic isoferritin‐inhibitory activity against normal granulocyte‐macrophage progenitor cellsBritish Journal of Haematology, 1983
- Identification of leukemia-associated inhibitory activity as acidic isoferritins. A regulatory role for acidic isoferritins in the production of granulocytes and macrophages.The Journal of Experimental Medicine, 1981
- Replacement of serum by insulin and transferrin supports growth and differentiation of the human promyelocytic cell line, HL-60Experimental Cell Research, 1980
- Suppressive Effect of Ferritin on in Vitro Lymphocyte FunctionBritish Journal of Haematology, 1979
- Continuous growth and differentiation of human myeloid leukaemic cells in suspension cultureNature, 1977
- A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye bindingAnalytical Biochemistry, 1976
- Cleavage of Structural Proteins during the Assembly of the Head of Bacteriophage T4Nature, 1970