The Liquid Chromatographic Separation of Metabolites of Benzo[a]pyrene with γ-Cyclodextrin as a Mobile Phase Additive
- 1 June 1995
- journal article
- research article
- Published by Taylor & Francis in Journal of Liquid Chromatography
- Vol. 18 (10) , 1909-1931
- https://doi.org/10.1080/10826079508013950
Abstract
The effects of γ-cyclodextrin as a mobile phase additive on the retention characteristics of fourteen metabolites of benzo[a]pyrene were investigated with reversed-phase liquid chromatography. It was possible to separate dihydrodiols, diones, and monohydroxyl-benzo[a]pyrenes as individual classes of metabolites. Also, with γ-cyclodextrin in the mobile phase, monohydroxyl-benzo[a]pyrene isomers that were very difficult to separate were readily separated. For example, 6-hydroxyl-benzo- [a]pyrene was separated from 12-hydroxyl-benzo[a]pyrene and 9-hydroxyl-benzo[a]pyrene was separated from 2-hydroxyl-benzo- [a]pyrene. The four tetrols were readily separated with shorter retention times using 4.0 mM of γ-cyclodextrin in a methanol-water (55:45) mobile phase. Overall, the results showed that γ-cyclodextrin was more effective in separating the metabolites than was β-cyclodextrin.Keywords
This publication has 22 references indexed in Scilit:
- Determination of DNA-benzo[a]pyrene adducts by high-performance liquid chromatography with laser-induced fluorescence detectionEnvironmental Science & Technology, 1992
- Cyclodextrins and their applications in analytical chemistryChemical Reviews, 1992
- Stereoselective metabolism of (—)-benzo [a]pyrene-7,8-diol by human lung microsomes and peripheral blood lymphocytes: effect of smokingCarcinogenesis: Integrative Cancer Research, 1992
- Metabolism of benzo[a]pyrene and (-)-trans-benzo[a]pyrene 7,8-dihydrodioi by freshly isolated hepatocytes from mirror carpCarcinogenesis: Integrative Cancer Research, 1991
- Metabolism of benzo[a]pyrene and (−)-trans-benzo[a]pyrene-7,8-dihydrodiol by freshly isolated hepatocytes of brown bullheadsChemico-Biological Interactions, 1990
- Cyclodextrin TechnologyPublished by Springer Nature ,1988
- The separation of the isomeric phenols of benzo(a)pyrene by high-performance liquid chromatographyAnalytical Biochemistry, 1981
- Improvement of reverse-phase high pressure liquid chromatographic resolution of benzo[a]pyrene metabolites using organic amines: Application to metabolites produced by fishAnalytical Biochemistry, 1979
- Separation of ten benzo(a)pyrene phenols by recycle high pressure liquid chromatography and identification of four phenols as metabolitesBiochemical Pharmacology, 1976
- Benzo[ a ]pyrene Metabolites: Efficient and Rapid Separation by High-Pressure Liquid ChromatographyScience, 1974