Serum-Deprivation Induces Cell Death of Rat Cultured Microglia Accompanied With Expression of Bax Protein.
Open Access
- 1 January 2000
- journal article
- Published by Elsevier in The Japanese Journal of Pharmacology
- Vol. 83 (4) , 351-354
- https://doi.org/10.1254/jjp.83.351
Abstract
Expression of Bcl−2 related proteins in rat microglial primary culture was examined.At relative low cell densities, serum deprivation caused cell death and nuclei condensation of cultured microglia.Expression of a pro−apoptotic protein, Bax, but not Bcl−2, was increased by the serum deprivation.SB203580, a specific inhibitor of p38MAPK, prevented both the serum deprivation−induced Bax expression and microglial death.Immunochemical staining showed that microglia expressing a high level of Bax were subjected to apoptosis−like cell death.These observations suggest that Bax expression underlies the apoptosis of cultured microglia after serum deprivation.Keywords
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