Abstract
A simple technique for mass rearing phlebotomine sand flies ( Phlebotomus papatasi and Lutzomyia longipalpis ) in the laboratory is described. Using this method, 4000–5000 adults can be produced weekly with minimal labor (2–3 h of care per day). This is the first report of mass production of sand flies, a group of insects that heretofore has been difficult to colonize and maintain under laboratory conditions.