Probing the secondary quinone (QB) environment in photosynthetic bacterial reaction centers by light‐induced FTIR difference spectroscopy
- 19 August 1991
- journal article
- Published by Wiley in FEBS Letters
- Vol. 288 (1-2) , 109-113
- https://doi.org/10.1016/0014-5793(91)81014-y
Abstract
The photoreduction of the secondary electron acceptor, QB, has been characterized by light‐induced Fourier transform infrared difference spectroscopy of Rb. sphaeroides and Rp. viridis reaction centers. The reaction centers were supplemented with ubiquinone (UQ10 or UQ0). The QB− state was generated either by continuous illumination at very low intensity or by single flash in the presence of redox compounds which rapidly reduce the photooxidized primary electron donor P+. This approach yields spectra free from P and P+ contributions making possible the study of the microenvironment of QB and QB−. Assignments are proposed for the C O vibration of QB− and tentatively for the C = O and C = C vibrations of QB.
Keywords
This publication has 16 references indexed in Scilit:
- Probing the primary quinone environment in photosynthetic bacterial reaction centers by light‐induced FTIR difference spectroscopyFEBS Letters, 1991
- Time-resolved FTIR spectroscopy of quinones in Rb. sphaeroides reaction centersBiochimica et Biophysica Acta (BBA) - Bioenergetics, 1990
- Characterization by FTIR spectroscopy of the photoreduction of the primary quinone acceptor QA in photosystem IIFEBS Letters, 1990
- A protein conformational change associated with the photoreduction of the primary and secondary quinones in the bacterial reaction centerFEBS Letters, 1990
- Investigation of models for photosynthetic electron acceptorsFEBS Letters, 1990
- Protonation of Quinones in Reaction Centers from Rb. sphaeroidesPublished by Springer Nature ,1990
- FTIR Studies of Light-Induced Intramolecular Processes on Crystals and Reconstituted Reaction Centers from Rhodopseudomonas viridisPublished by Springer Nature ,1990
- Structures and fundamental vibrations of p-benzoquinone and p-benzosemiquinone radical anion from ab initio calculationsThe Journal of Physical Chemistry, 1986
- Electron nuclear double resonance of semiquinones in reaction centers of Rhodopseudomonas sphaeroidesBiochimica et Biophysica Acta (BBA) - Bioenergetics, 1985
- Secondary electron transfer in reaction centers of Rhodopseudomonas sphaeroides. Out-of-phase periodicity of two for the formation of ubisemiquinone and fully reduced ubiquinoneBiochimica et Biophysica Acta (BBA) - Bioenergetics, 1977