Determination of Histamine and Its Metabolic Products in the Pig Gastric Mucosa

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Abstract
This paper describes analytical techniques for quantitative and qualitative determination of histamine and its metabolites (Nτ-methylhistamine, Nα-methylhistamine and Nα, Nα-dimethylhistamine) in the pig gastric mucosa. These metabolites and Nα-acetylhistamine, imidazolyl-4-acetic acid and Nτ-methylimidazolylacetic acid were synthesized as reference compounds and analyzed by using the dansylation technique. TLC of dansylated mucosal extracts in various solvents {in situ fluorescence measurements) in combination with TLC/IR and TLC/MS transfer technique demonstrated the presence of histamine. The only metabolite was Nτ-methyl-histamine.