Does pretreatment by rhodamine 6-G affect the mitochondrial composition of fusion-derived Nicotiana cybrids?
- 1 June 1986
- journal article
- research article
- Published by Springer Nature in Plant Cell Reports
- Vol. 5 (3) , 227-230
- https://doi.org/10.1007/bf00269126
Abstract
Rhodamine-6G(R6G), a lipophilic dye which degrades mammalian mitochondria, was shown to arrest the division of Nicotiana protoplasts. When albino recipient-protoplasts were treated with R6G and fused with X-irradiated (green) donor- protoplasts, only green cybrid plants were obtained. The mtDNA of the cybrids was analyzed by Southern-blot hybridization. We found that cybrids which resulted from N. rustica (donor) protoplasts, fused with R6G-treated albino protoplasts, had only parental-type mtDNA. When another donor, with N. undulata mtDNA, was used, most of the resulting cybrids contained non-parental mtDNA. Only one cybrid (out of 12) had N. undulata -type (donor) mtDNA.Keywords
This publication has 18 references indexed in Scilit:
- Organelle transferPublished by Elsevier ,1986
- Novel alloplasmic Nicotiana plants by “donor-recipient” protoplast fusion: cybrids having N. tabacum or N. sylvestris nuclear genomes and either or both plastomes and chondriomes from alien speciesMolecular Genetics and Genomics, 1984
- Generation of heteroplastidic Nicotiana cybrids by protoplast fusion: analysis for plastid recombinant typesTheoretical and Applied Genetics, 1984
- Mitochondria control expression of a murine cell surface antigenNature, 1983
- Cybrids containing mixed and sorted-out chloroplasts following interspecific somatic fusions in NicotianaTheoretical and Applied Genetics, 1983
- Novel composition of mitochondrial genomes in Petunia somatic hybrids derived from cytoplasmic male sterile and fertile plantsMolecular Genetics and Genomics, 1983
- Extensive rearrangements in the mitochondrial DNA in somatic hybrids of Nicotiana tabacum and Nicotiana knightianaMolecular Genetics and Genomics, 1981
- Elimination of mitochondrial elements and improved viability in hybrid cellsSomatic Cell and Molecular Genetics, 1981
- The use of cytoplasmic streptomycin resistance: Chloroplast transfer from Nicotiana tabacum into Nicotiana sylvestris, and Isolation of their somatic hybridsMolecular Genetics and Genomics, 1980
- A Revised Medium for Rapid Growth and Bio Assays with Tobacco Tissue CulturesPhysiologia Plantarum, 1962