Purity of Staphylococcal -haemolysin obtained by three different procedures

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Abstract
Three methods available for the purification of staphylococcal .delta.-hemolysin were compared as to the purity and identity of the product obtained. None yielded a pure preparation .delta.-hemolysin; 1 of the 3 preparations did not contain demonstrable .delta.-hemolysin when tested electrophoretically, but it contained DNase, penicillinase, phosphatase and .alpha.-hemolysin. The 2nd preparation had .delta.-hemolysin activity and was free of .alpha.-hemolysin, but it contained lipase, egg-yolk factor, esterase, DNase, penicillinase, phosphatase and hyaluronidase. The 3rd preparation contained all of the products mentioned above except phosphatase, and it also contained .alpha.-hemolysin, staphylokinase, lysozyme and caseinase. These findings are discussed with special reference to the requirement for criteria of purity in work with staphylococcal products.