Genetic dissection of Drosophila adenylate cyclase.
- 1 September 1985
- journal article
- Published by Proceedings of the National Academy of Sciences in Proceedings of the National Academy of Sciences
- Vol. 82 (17) , 5992-5996
- https://doi.org/10.1073/pnas.82.17.5992
Abstract
The Drosophila learning mutant rutabaga is missing calcium/calmodulin activation of adenylate cyclase (EC4.6.1.1). The mutation was mapped at a finer resolution to X chromosome bands 12F5-7. By comparing wild-type and mutant cyclase activities, the relative responsiveness of the calcium-sensitive and calcium-insensitive components to different ligands could be determined; the calcium-sensitive fraction of the total cyclase activity was significantly less responsive to guanyl nucleotides and monoamines. The results suggest that the component of cyclase activity that is stimulated by calcium/calmodulin, possibly a genetically distinct catalytic subunit, is not coupled to the G subunit or the G subunit/monoamine receptor complex.Keywords
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