Three sequence-specific endonucleases fromEscherichia coliRFL47
- 19 September 1983
- journal article
- Published by Wiley in FEBS Letters
- Vol. 161 (2) , 213-216
- https://doi.org/10.1016/0014-5793(83)81010-2
Abstract
The characterization of the new restriction enzyme Eco47III recognizing a hexanucleotide palindromic sequence 5'AGC↓GCT and cleaving, as indicated by the arrow, is reported. It was isolated from Escherichia coli strain RFL47. Another two specific endonuclease Eco47I (isoschizomer of AvaII) and Eco47II (isoschizomer of AsuI) were also found in this strain. There are two Eco47III recognition sites on λ DNA at 20997 and 37060 basepairs. The central Eco47III fragment can be replaced by a cloned fragment in λ vector mutant in tR2 gene; i.e., λ gKeywords
This publication has 12 references indexed in Scilit:
- A new sequence-specific endonuclease fromGluconobacter suboxydansFEBS Letters, 1983
- The effect of site specific methylation on restriction endonuclease cleavage (update)Nucleic Acids Research, 1983
- The bacteriopbage λ O replication protein: isolation and characterization of the amplified initiatorNucleic Acids Research, 1983
- Nucleotide sequence of bacteriophage λ DNAJournal of Molecular Biology, 1982
- A new restriction endonuclease fromCitrobacter freundiiNucleic Acids Research, 1982
- Identification of palindromic sequences recognized by restriction endonucleases, as based on the tabularized sequencing data for seven viral and plasmid DNAsGene, 1980
- Complete Nucleotide Sequence of the Escherichia coli Plasmid pBR322Published by Cold Spring Harbor Laboratory ,1979
- Site specificity and chromatographic properties of E. COLI K12 and EcoRII DNA‐cytosine methylasesFEBS Letters, 1978
- The cleavage site of the restriction endonuclease Ava IINucleic Acids Research, 1978
- DNA sequence analysis: a general, simple and rapid method for sequencing large oligodeoxyribonucleotide fragments by mappingNucleic Acids Research, 1974