RIBOSOMES IN NORMAL AND GIANT PLACENTAE

Abstract
Progesterone and oestrogen administered to rats ovariectomized during gestation will successfully maintain pregnancy. However, the altered steroid environment created by ovariectomy may result in enlarged placentae (Carpent, 1962; Csapo & Wiest, 1969; Hooverman, Meyer & Wolf, 1970) without influencing the decidua basalis (Stewart & Leathem, 1968). Normal placental growth involves cell division and the synthesis of protein and nucleic acids until day 17. At this time DNA synthesis stops but RNA continues to accumulate (Winick & Noble, 1966). When giant placentae were induced in steroid-treated ovariectomized rats, protein and nucleic acid content exceeded the normal (Leathem, Stewart & Sweki, 1970). This finding prompted an examination of the quantity and distribution of ribosomes. Sprague—Dawley rats weighing 200 g were mated and day 1 of pregnancy determined by the presence of sperm in the vaginal smear. Oestrone 0·5 μg) and progesterone (4·0 mg) were injected s.c. daily from days 3–20 in intact