Large subunit ribulosebisphosphate carboxylase messenger RNA from Euglena chloroplasts.
- 1 March 1976
- journal article
- Published by Proceedings of the National Academy of Sciences in Proceedings of the National Academy of Sciences
- Vol. 73 (3) , 722-726
- https://doi.org/10.1073/pnas.73.3.722
Abstract
RNA from wild-type Euglena, aplastidic mutant cells, and purified chloroplasts was chromatographed on oligo(dT)-cellulose at 4 degrees. The poly(A)-containing and poly(A)-lacking fractions from each were then tested in a cell-free protein-synthesizing system from wheat germ for their abilities to specifically stimulate synthesis of large subunit ribulosebisphosphate carboxylase [EC 4.1.1.39; 3-phospho-D-glycerate carboxy-lyase (dimerizing)]. The large subunit polypeptide (59,000 molecular weight) was identified in the in vitro reaction by two-dimensional electrophoresis involving isoelectric focusing and size filtration on polyacrylamide gels. Template activity for the large subunit was detected in wild-type cells but not in aplastidic mutant strains; it was highly enriched in the isolated chloroplasts. This messenger was present only in the poly(A)-lacking RNA fraction, where it constituted the most prominent template species of chloroplast RNA. The large subunit message was freed of considerable non-messenger RNA contamination and localized to the 10-20S fraction by sucrose gradient centrifugation.Keywords
This publication has 23 references indexed in Scilit:
- Major mRNA species from spinach chloroplasts do not contain poly(A)Nature, 1975
- A Film Detection Method for Tritium‐Labelled Proteins and Nucleic Acids in Polyacrylamide GelsEuropean Journal of Biochemistry, 1974
- Poly(A)-associated RNA in plantsBiochimica et Biophysica Acta (BBA) - Nucleic Acids and Protein Synthesis, 1974
- Efficient Translation of Tobacco Mosaic Virus RNA and Rabbit Globin 9S RNA in a Cell-Free System from Commercial Wheat GermProceedings of the National Academy of Sciences, 1973
- Purification of Biologically Active Globin Messenger RNA by Chromatography on Oligothymidylic acid-CelluloseProceedings of the National Academy of Sciences, 1972
- Polyadenylic Acid Sequences in the Heterogeneous Nuclear RNA and Rapidly-Labeled Polyribosomal RNA of HeLa Cells: Possible Evidence for a Precursor RelationshipProceedings of the National Academy of Sciences, 1971
- A Polynucleotide Segment Rich in Adenylic Acid in the Rapidly-Labeled Polyribosomal RNA Component of Mouse Sarcoma 180 Ascites CellsProceedings of the National Academy of Sciences, 1971
- Isolation and characterization of Euglena gracilis cytoplasmic and chloroplast ribosomes and their ribosomal RNA componentsBiochimica et Biophysica Acta (BBA) - Nucleic Acids and Protein Synthesis, 1969
- Polysome formation with tobacco mosaic virus RNA.Proceedings of the National Academy of Sciences, 1968
- Studies of chloroplast development in EuglenaJournal of Molecular Biology, 1965