In vitro non-thrombogenicity of a thrombin-substrate-immobilized polymer surface by the inhibition of thrombin activity
- 1 January 1991
- journal article
- Published by Taylor & Francis in Journal of Biomaterials Science, Polymer Edition
- Vol. 2 (2) , 123-138
- https://doi.org/10.1163/156856291x00115
Abstract
Derivatives of thrombin substrate were synthesized and immobilized on a poly(acrylic acid)-grafted polyurethane film. The carboxyl terminal of the thrombin substrate peptide should be blocked for a higher inhibitory effect of the thrombin activity. Immobilization of the thrombin substrate peptide enhanced adsorption and inactivation of thrombin on the polymer film to prolong the time for fibrin network formation, and suppressed adhesion and deformation of platelets on the film. Consequently, in vitro thrombus formation on the polymer film was strongly suppressed.Keywords
This publication has 14 references indexed in Scilit:
- Antithrombogenic surfaces: Characterization and bioactivity of surface immobilized PGE1‐heparin conjugateJournal of Biomedical Materials Research, 1989
- Platelet adhesion onto protein‐coated and uncoated polyetherurethaneurea having tertiary amino groups in the substituents and its derivativesJournal of Biomedical Materials Research, 1989
- Platelet activation by thrombin in the absence of the high-affinity thrombin receptorBiochemistry, 1988
- Highly sensitive peptide‐4‐methylcoumaryl‐7‐amide substrates for blood‐clotting proteases and trypsinEuropean Journal of Biochemistry, 1988
- Antithrombogenic Heparin-Bound PolyurethanesJournal of Biomaterials Applications, 1987
- Synthesis and antithrombogenicity of anionic polyurethanes and heparin‐bound polyurethanesJournal of Biomedical Materials Research, 1986
- Structure of human factor VIIINature, 1984
- COVALENT STRUCTURE OF FIBRINOGENAnnals of the New York Academy of Sciences, 1983
- Platelet plasma membrane glycoproteins Identification of a proteolytic substrate for thrombinBiochemical and Biophysical Research Communications, 1977
- Kinetics of the thrombin-induced release of calcium(II) by plateletsBiochemistry, 1973