Nucleotide sequence at the termini of the DNA of Bacillus subtilis phage phi 29.
- 1 March 1981
- journal article
- research article
- Published by Proceedings of the National Academy of Sciences in Proceedings of the National Academy of Sciences
- Vol. 78 (3) , 1446-1450
- https://doi.org/10.1073/pnas.78.3.1446
Abstract
Phage .vphi.29 DNA cannot be phosphorylated with polynucleotide kinase and [.gamma.-32P]ATP because of the presence of a viral protein covalently linked to the 5'' termini. The 5'' ends can be made susceptible to phosphorylation by treatment with alkali and alkaline phosphatase. Restriction fragments Hpa II C and Hpa II F, corresponding to the right and left ends of .vphi.29 DNA, respectively, were labeled at the 5'' ends with polynucleotide kinase and [.gamma.-32P]ATP or at the 3'' ends with terminal transferase and [.alpha.-32P]ATP or [.alpha.-32P]cordycepin 5''-triphosphate. After a secondary cleavage of the labeled fragments, the sequence of the first 150-180 nucleotides at the termini of .vphi.29 DNA was determined by the Maxam and Gilbert method. The ends of .vphi.29 DNA are flush and a 6-nucleotides-long inverted terminal repetition was found. The functional implications of the sequences determined are discussed.This publication has 56 references indexed in Scilit:
- Specific cleavage of the terminal protein from the adenovirus 5 DNA under the condition of single‐strand scission by nuclease S1FEBS Letters, 1979
- Sequence of inverted terminal repetitions from different adenoviruses: Demonstration of conserved sequences and homology between SA7 termini and SV40 DNACell, 1979
- Nucleotide sequence at the inverted terminal repetition of adenovirus type 2 DNABiochemical and Biophysical Research Communications, 1979
- The nucleotide sequences at the termini of adenovirus-2 DNAJournal of Molecular Biology, 1979
- RNA Polymerase binding sites and transcription map of the DNA of Bacillus subtilis phage φ29Journal of Molecular Biology, 1979
- Characterization of a protein covalently linked to the 5′ termini of the DNA of Bacillus subtilis phage φ29Journal of Molecular Biology, 1978
- The structure of replicating adenovirus 2 DNA moleculesCell, 1977
- Determinant of cistron specificity in bacterial ribosomesNature, 1975
- Palindromic base sequences and replication of eukaryote chromosome endsNature, 1974
- Polynucleotide. CXXIV. Reversal of bacteriophage T4 induced polynucleotide kinase actionBiochemistry, 1973