Genetically modified rice seeds accumulating GLP‐1 analogue stimulate insulin secretion from a mouse pancreatic beta‐cell line
Open Access
- 19 January 2005
- journal article
- Published by Wiley in FEBS Letters
- Vol. 579 (5) , 1085-1088
- https://doi.org/10.1016/j.febslet.2004.12.082
Abstract
Glucagon‐like peptide‐1 (7‐36) amide (GLP‐1) is the most potent physiological insulinotropic hormone in humans. We produced large amounts of a GLP‐1 analogue, [Ser8, Gln26, Asp34]‐GLP‐1, which is resistant to trypsin‐digestion, as part of a chimeric rice seed storage protein, a 26 kDa globulin, in genetically modified rice seeds. Junction sites between GLP‐1 analogue and globulin were replaced by tryptic cleavage sites. The highest level of GLP‐1 analogue accumulation was ≈20–50 μg per seed. We found that GLP‐1 analogue derived from trypsin‐digested genetically modified rice seeds stimulated insulin secretion from a mouse pancreatic beta‐cell line, MIN6.Keywords
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