Lineages of Acidophilic Archaea Revealed by Community Genomic Analysis
- 22 December 2006
- journal article
- other
- Published by American Association for the Advancement of Science (AAAS) in Science
- Vol. 314 (5807) , 1933-1935
- https://doi.org/10.1126/science.1132690
Abstract
Novel, low-abundance microbial species can be easily overlooked in standard polymerase chain reaction (PCR)–based surveys. We used community genomic data obtained without PCR or cultivation to reconstruct DNA fragments bearing unusual 16S ribosomal RNA (rRNA) and protein-coding genes from organisms belonging to novel archaeal lineages. The organisms are minor components of all biofilms growing in pH 0.5 to 1.5 solutions within the Richmond Mine, California. Probes specific for 16S rRNA showed that the fraction less than 0.45 micrometers in diameter is dominated by these organisms. Transmission electron microscope images revealed that the cells are pleomorphic with unusual folded membrane protrusions and have apparent volumes of <0.006 cubic micrometer.Keywords
This publication has 24 references indexed in Scilit:
- Community Genomics Among Stratified Microbial Assemblages in the Ocean's InteriorScience, 2006
- Detection and Isolation of Ultrasmall Microorganisms from a 120,000-Year-Old Greenland Glacier Ice CoreApplied and Environmental Microbiology, 2005
- Acetoclastic and hydrogenotrophic methane production and methanogenic populations in an acidic West‐Siberian peat bogEnvironmental Microbiology, 2004
- Contribution of SAR11 Bacteria to Dissolved Dimethylsulfoniopropionate and Amino Acid Uptake in the North Atlantic OceanApplied and Environmental Microbiology, 2004
- Environmental Genome Shotgun Sequencing of the Sargasso SeaScience, 2004
- Community structure and metabolism through reconstruction of microbial genomes from the environmentNature, 2004
- Extremely Acidophilic Protists from Acid Mine Drainage Host Rickettsiales -Lineage Endosymbionts That Have Intervening Sequences in Their 16S rRNA GenesApplied and Environmental Microbiology, 2003
- Microbial communities in acid mine drainageFEMS Microbiology Ecology, 2003
- Bacterial Rhodopsin: Evidence for a New Type of Phototrophy in the SeaScience, 2000
- Ultrastructure of the Hyperthermophilic Archaeon Pyrodictium abyssiJournal of Structural Biology, 1995