Studies on the conformation of human serum high-density lipoproteins HDL2 and HDL3.

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Abstract
-"The effect of lipid removal on the conformation of the apoproteins [alpha]P2 and [alpha]P3, of the 2 high-density lipoprotein subclasses, HDL2 (d 1.063-1.120) and HDL3 (1.120-1.21), was studied using the techniques of optical rotatory dispersion and viscometry. The data indicated that either in their native state (i.e., in association with liplds) or upon dellpidation, [alpha]P2 and [alpha]P3 had a high [alpha]-helical content, estimated between 60 to 70% for the native state and 55 to 65% for the delipldated forms. The helical structure, which was disrupted by the action of urea or guanidlne[middot]HCl, was to a large extent restored upon removal of the denaturing agents by dialysis. The data were interpreted as consistent with the view that lipids do not participate in the secondary structure HDL apoproteins, but act as bridges between the various apoproteln subunlts.
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