Separation of Low Molecular Weight RNA Species by High-Speed Gel Filtration

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Abstract
By employing a high-speed gel filtration method using a column of silica-based aqueous gel, TSK-GEL G 3000 SW, the 3 low MW RNA species, 5.8S and 5S rRNA and tRNA, were clearly separated from each other and from high MW RNA species in 200 mM sodium phosphate buffer, pH 7.0, containing 0.1% sodium dodecyl sulfate. Excellent reproducibility and resolution were obtained under these conditions, .apprx. 30 min being required for a single run. Furthermore, the 5.8S rRNA from rat liver cells was eluted at a different position compared with that of the yeast, Saccharomyces cerevisiae, although they have the same nucleotide chain length but a different sequence. The 5.8S rRNA from the cellular slime mold, Dictyostelium discoideum, was eluted at the same position as that of yeast.