Structural and Phylogenetic Analysis of the γ-Actin Encoding Gene from the Penicillin-Producing Fungus Penicillium chrysogenum
- 1 February 2001
- journal article
- research article
- Published by Springer Nature in Current Microbiology
- Vol. 42 (2) , 117-121
- https://doi.org/10.1007/s0028403338
Abstract
The nucleotide sequence of a 2994-bp genomic fragment, including the γ-actin encoding gene from Penicillium chrysogenum, has been determined, showing an open reading frame (ORF) of 1756 bp interrupted by five introns with fungal consensus splice-site junctions. The 5′ untranslated region contains a consensus TATA box, five CAAT motifs, and two large pyrimidine stretches. The predicted protein (375 amino acids) revealed high identity to γ-actins from fungi (>90%), and gene phylogenies support the grouping of P. chrysogenum actin close to those from the majority of the filamentous fungi. The actA gene is present as a single copy in the genome of P. chrysogenum, and its expression is constitutive during penicillin fermentation, showing a single 1.4-kb transcript.Keywords
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