[Evaluation of a new method for antifungal drugs susceptibility testing to yeasts].

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Abstract
We compared the Etest® with a broth microdilution method (FP panel®), performed according to the National Committee for modified Clinical Laboratory Standards (NCCLS) document M27-P guidelines, for determining the MICs of 81 clinical isolates of yeasts (7 Candida albicans, 8 Candida glabrata, 10 Candida parapsilosis, 6 Pichia anomala, 10 Candida tropicalis, 4 Candida guilliermondii, 4 Candida krusei, 6 Trichosporon cutaneum, 5 Candida ciferrii, 3 Candida famata, 4 Candida norvegensis, 2 Rhodotorula rubra, 3 Candida lusitaniae, 2 Candida curvata, 1 Candida inconspicua, 1 Candida intermedia, 1 Candida colliculosa, 1 Cryptococcus spp, 1 Tricosporon capitatum, 1 Pichia ohmeri, 1 Saccharomyces cerevisiae). The Etest® results for 6 ATCC standard strains correlated well with reference MICs except those of flucytosine (5-FC) for C. krusei, which tended to be 1 to 2 log2 dilution higher than the MIC range determined by NCCLS guidelines. However, the best agreement between the results for clinical isolates was seen with 5-FC (100% agreement [Within ×2 log2 dilutions] between the results of the two methods). There was a 91.4% agreement between the results of the two methods with amphotericin B (Etest® MICs tended to be 1 to 2 log2 dilution lower than those of the FP panel). The Etest® results with litraconazole for clinical isolates except C. tropicalis were similar to MICs of the FP panel® (Etest® for C. tropicalis showed 1 to 2 log2 dilution lower than FP panel®). Also, the Etest® results with fluconazole for clinical isolates except C. tropicalis were similar of 1 log2 dilution higher than MICs of the FP panel® (Etest® for C. tropicalis showed more than 2 log2 dilution lower thas FP panel®). These results showed a good level of overall agreement between the Etest® method and the broth microdilution test (FP panel®). Since the Etest® is a less laborintensive and much simpler method, it appears to be a useful procedure for testing the susceptibility of yeasts to antifungal agents.

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